Coding

Part:BBa_K2443044:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-05)


Cys RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 859


Design Notes

Codon optimized for use in Escherichia coli.



Source

Genomic cysS gene sequence obtained from: Escherichia coli str. K-12 substr. MG1655, complete genome NCBI Reference Sequence: NC_000913.3


References